What is BPC-157 + TB-500 (Wolverine Blend)?
The blend has no CAS number, formula or molecular weight, because it is a mixture of two peptides rather than a single chemical entity, and our dataset records those fields empty. The component records are complete. BPC-157 is CAS 137525-51-0, formula C62H98N16O22, average molecular weight 1419.53, with the fifteen-residue sequence H-Gly-Glu-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val-OH. TB-500 is CAS 885340-08-9, formula C38H68N10O14, average molecular weight 889.02, the seven-residue acetylated sequence Ac-Leu-Lys-Lys-Thr-Glu-Thr-Gln-OH.
Equal masses are not equal amounts. Dividing 10 mg by each molecular weight gives roughly 7.0 micromoles of the pentadecapeptide against 11.2 micromoles of the heptapeptide. Work in molar terms starts from that arithmetic, not the label masses.
The two components are studied for different reasons. BPC-157 appears in rodent models of gastrointestinal and soft tissue repair, centring on nitric oxide pathways and growth factor expression. TB-500 is the Leu-Lys-Lys-Thr-Glu-Thr-Gln motif that forms the actin-binding site of thymosin beta-4, and the sequestration and migration work cited alongside it was done on that 43-residue parent. Whether combining the two produces anything beyond the sum of the separate observations is not established.
Reconstitution & handling
Both components are highly water soluble, so sterile or bacteriostatic water clears the cake with gentle swirling. Add diluent down the vial wall and do not shake, since either will foam if agitated. Concentration arithmetic is done per component: the vial holds two 10 mg parts, so the diluent volume gives a figure for each, and both are recorded rather than one combined number.
Storage & stability
Store the sealed vial at -20 °C, desiccated and protected from light. Once reconstituted, keep it at 2 to 8 °C in single-use aliquots, avoiding freeze-thaw. Neither component carries a methionine or a cysteine, so oxidation is not the named degradation route here. Moisture while dry and slow hydrolytic change in solution are the routes that matter, and a mixture is only as stable as its least stable member.
How it's tested
A blend is released on component-resolved analysis. Reversed-phase HPLC at 214 and 220 nm separates the two peptides, and purity is reported per peak rather than as one figure. Mass spectrometry confirms both identities against the theoretical average masses of 1419.53 and 889.02. Net peptide content is reported per component so the 10 mg plus 10 mg fill can be verified, and a lot-matched certificate is available on request.