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Research Compound Reference

P-21

P-21 is a small peptidomimetic derived from an active fragment of ciliary neurotrophic factor and studied in rodent neurogenesis models. It is supplied as a lyophilized powder in a sealed vial for in vitro and preclinical research only.

Molecular profile of P-21
Also known asP021; neurogenic peptidomimetic
Amino Acid Count6
SequenceAc-Asp-Gly-Gly-Leu-Ala-Gly-NH2 (CNTF-derived hexapeptide)
Purity≥99% by HPLC · COA available
Physical FormLyophilized powder

What is P-21?

P-21, carried in our catalog under the synonym P021, is described in the literature as a peptidomimetic developed from an active fragment of ciliary neurotrophic factor. Our molecular dataset holds no CAS number, formula, molecular weight or sequence for this entry, so none of those values are quoted here; a batch's figures are stated on its certificate of analysis.

What can be said without those records is that the compound belongs to the peptidomimetic class rather than the straightforward peptide class. Molecules of that kind take a short active stretch of a parent protein and cap or replace parts of it with non-peptide chemistry, for resistance to peptidase cleavage and better membrane behaviour.

Research interest centres on neurotrophic signalling. Rodent work has used the compound to model adult hippocampal neurogenesis and to probe brain-derived neurotrophic factor expression, with reported inhibition of leukaemia inhibitory factor signalling proposed as one way a fragment can act in the opposite direction to the full-length cytokine. The literature base is small, the mechanism is not independently settled, and comparative work against other neurotrophic tool compounds is sparse.

Reconstitution & handling

Sterile or bacteriostatic water is the usual starting diluent. Because our records carry no solubility data for this entry, add a small volume first and check that the solution runs clear, with no haze or particulate, before adding the rest. Swirl, do not shake. Concentration is the labelled mass divided by the total diluent volume.

Storage & stability

The sealed lyophilized vial belongs at -20 °C, desiccated and shielded from light, the condition the dry cake is packaged for. Once in solution, hold at 2 to 8 °C and divide into single-use aliquots rather than returning a working stock to the freezer. Where a degradation profile is not well described, as here, short solution hold times and fresh preparation are the conservative choices.

How it's tested

Release testing runs reversed-phase HPLC at 214 and 220 nm against a main-peak purity threshold of 99 percent or better. Identity is confirmed by mass spectrometry, the observed mass compared against the theoretical mass calculated for the batch structure rather than any value quoted here. Net peptide content and water are reported separately, and the lot-matched certificate of analysis is available on request.

Frequently asked questions

Why does this page not list a molecular weight?

Our molecular dataset holds no CAS, formula, weight or sequence for this handle, and we do not publish figures we cannot source internally. The shipped lot's certificate of analysis carries them.

Is P-21 the same as the parent neurotrophic factor?

No. The literature describes it as a small mimetic derived from an active fragment, not the full-length cytokine, and reports of opposing effects on some signalling arms are part of why it is studied.

What is the main open question in the literature?

Mechanism. The proposed action on leukaemia inhibitory factor signalling and downstream neurotrophin expression rests on few rodent reports and has not been broadly replicated.

Shop lot-tested P-21

Third-party tested to ≥99% purity, with analytical documentation available.

View product →Reconstitution calculator
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This reference describes the compound's chemistry and analytical properties for laboratory research use only. It is not medical advice; the product is not a drug, supplement, or cosmetic and is not for human or veterinary consumption.